Gradient materials are loaded while the rotor is at rest, the flow is stopped and a linear density gradient is formed while the rotor is accelerating to a set speed. Following a reorientation phase the gradient transitions from horizontal to vertical in the rotor. After this transition occurs then the rotor can be accelerated to set speed while in a continuous flow operation.
|1||The density gradient is loaded into the rotor while it is at rest.|
|2||As the rotor is gradually accelerated, the gradient reorients vertically inside the rotor assembly.|
|3||Once at the required operating speed, sample fluid is pumped into the rotor on a continuous flow basis.|
|4||The sample particles sediment radially into the linear density gradient. The particles band (iso-pycnically) in cylindrical zones where the gradient density equals a particle's buoyant density.|
|5||At the end of the run, the rotor is decelerated to rest.|
|6||During deceleration the gradient reorients itself to the original position without loss of resolution in the particle bands.|
|7||The banded particles are now ready to be unloaded. Fractions are collected using the Automated Fluid Handling System.|